Fall 2015

Seminars are held on Thursdays in Cullimore Hall. For questions about the seminar schedule, please contact Antai Wang.


Date: November 5, 2015

Speaker: Mingyao Li
Department of Biostatistics and Epidemiology,
University of Pennsylvania

University Profile

Title: "Modeling Transcriptomic Variations in RNA Sequencing"

Abstract:

RNA sequencing (RNA-seq) allows an unbiased survey of the entire transcriptome in a high-throughput manner. It has rapidly replaced microarrays as the major platform for transcriptomics studies. Analysis of RNA-seq data, however, is challenging because various biases present in RNA-Seq data can complicate the analysis, and if not appropriately corrected, will affect gene expression estimation and downstream modeling. In this talk, I will present several statistical/computational issues related to the analysis of transcriptomic variations in RNA-seq data. I will first present PennSeq, a method that we recently developed for isoform-specific gene expression estimation. I will then discuss methods for detecting differential alternative splicing. I will show simulation results as well as examples from real RNA-seq studies.